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Tutorial Pgem T Easy Vector

This post categorized under Vector and posted on August 12th, 2019.
PGEM Vector: Tutorial Pgem T Easy Vector

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PGEM-T Easy Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI EcoRI and NotI sites.The pGEM -T and pGEM -T Easy Vector Systems include a 2X Rapid Ligation Buffer for ligation of PCR products. Reactions using this buffer may be incubated for 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation.pGEM-T Parental vector for TA cloning of PCR products. The insertion site is flanked by BstZI sites. This vector is also known as pGEM5Zf().

Close This website uses cookies to ensure you get the best experience. By continuing to use this site you agree to the use of cookies.The pGEM-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine in a template-independent fashion to the 3-ends of the amplified fragments.Comparing Cloning Efficiency of the pGEM-T and pGEM-T Easy Vectors to the TOPO TA Cloning Vectors Citations Roles of 14-3-3 and in regulation of the glucocorticoid receptor transcriptional activation and hepatic gluconeogenesis.

pGEM -T Easy Vector SystempGEM -T Vector SystemEcoRINotI

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