# Difference In Egfp Expression In Mouse Es Cells Transfected With Two Gene And Three Genefig

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Vector and posted on January 11th, 2019.

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3.2. EGFP Activity in Stably Transfected Cells In order to know if the minisatellite would be able to affect transcription in vivo we determined whether the reporter gene was transcriptionally active in a chromatin environment.Similarly compared with non-transfected cells a characteristic band of 53 kDa was observed by western blotting in MCF-7 cells transfected with LV-VEGF165-EGFP whose size was consistent with that of the VEGF165-EGFP fusion protein indicating that the VEGF165-EGFP fusion gene in the recombinant LV could be expressed following transfection into MCF-7 cells.15.10.2012 Three conditions were tested in this experiment. The experimental group and the positive control group consisted of 293T cells transfected with pEGFP-N1-DTA and pEGFP-N1 respectively and the third group was the negative control which was incubated with complete medium alone.

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